JUMP TO

2016

Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine
Lobo NC, Gedye C, Apostoli AJ, Brown KR, Paterson J, Stickle N, Robinette M, Fleshner N, Hamilton RJ, Kulkarni G, Zlotta A, Evans A, Finelli A, Moffat J, Jewett MA, Ailles L.  BMC Cancer. 2016 Jul 16;16:485. doi: 10.1186/s12885-016-2539-z. Pubmed


The SAGA deubiquitination module promotes DNA repair and class switch recombination through ATM and DNAPK-mediated γH2AX formation
Ramachandran S, Haddad D, Li C, Le MX, Ling AK, So CC, Nepal RM, Gommerman JL, Yu K, Ketela T, Moffat J, Martin A.  Cell Rep. 2016 May 17;15(7):1554-65. Pubmed


BAGEL: a computational framework for identifying essential genes from pooled library screens
Hart T, Moffat J.  BMC Bioinformatics. 2016 Apr 16;17:164. Pubmed


Inhibition of SCF ubiquitin ligases by engineered ubiquitin variants that target the Cul1 binding site on the Skp1-F-box interface
Gorelik M, Orlicky S, Sartori MA, Tang X, Marcon E, Kurinov I, Greenblatt JF, Tyers M, Moffat J, Sicheri F, Sidhu SS.  Proc Natl Acad Sci USA. 2016 Mar 29;113(13):3527-32. Pubmed


System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes
Zhang W, Wu KP, Sartori MA, Kamadurai HB, Ordureau A, Jiang C, Mercredi PY, Murchie R, Hu J, Persaud A, Mukherjee M, Li N, Doye A, Walker JR, Sheng Y, Hao Z, Li Y, Brown KR, Lemichez E, Chen J, Tong Y, Harper JW, Moffat J, Rotin D, Schulman BA, Sidhu SS.  Mol Cell. 2016 Apr 7;62(1):121-36. Pubmed


Pooled screening for antiproliferative inhibitors of protein-protein interactions
Nim S, Jeon J, Corbi-Verge C, Seo MH, Ivarsson Y, Moffat J, Tarasova N, Kim PM.  Nat Chem Biol. 2016 Apr;12(4):275-81. Pubmed


Cytokinetic effects of Wee1 disruption in pancreatic cancer
Chang Q, Chandrashekhar M, Ketela T, Fedyshyn Y, Moffat J, Hedley D.  Cell Cycle. 2016 Feb 16;15(4):593-604. Pubmed


Automated inference procedure for the determination of cell growth parameters
Harris EA, Koh EJ, Moffat J, McMillen DR.  Phys Rev E. 2016 Jan;93(1):012402. Pubmed


Functional genomic landscape of human breast cancer drivers, vulnerabilities and resistance
Marcotte R, Sayad A, Brown KR, Sanchez-Garcia F, Reimand J, Haider M, Virtanen C, Bradner JE, Bader GD, Mills GB, Pe'er D, Moffat J, Neel BG.  Cell. 2016 Jan 14;164(1-2):293-309. Pubmed


SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination
Zhao DY, Gish G, Braunschweig U, Li Y, Ni Z, Schmitges FW, Zhong G, Liu K, Li W, Moffat J, Vedadi M, Min J, Pawson TJ, Blencowe BJ, Greenblatt JF.  Nature. 2016 Jan 7;529(7584):48-53. Pubmed


Identification of RNF168 as a PML nuclear body regulator
Shire K, Wong AI, Tatham MH, Anderson OF, Ripsman D, Gulstene S, Moffat J, Hay RT, Frappier L.  J Cell Sci. 2016 Feb 1;129(3):580-91. Pubmed


2015

Genome-wide CRISPR-Cas9 screens reveal loss of redundancy between PKMYT1 and WEE1 in glioblastoma stem-like cells
Toledo CM, Ding Y, Hoellerbauer P, Davis RJ, Basom R, Girard EJ, Lee E, Corrin P, Hart T, Bolouri H, Davison J, Zhang Q, Hardcastle J, Aronow BJ, Plaisier CL, Baliga NS, Moffat J, Lin Q, Li XN, Nam DH, Lee J, Pollard SM, Zhu J, Delrow JJ, Clurman BE, Olson JM, Paddison PJ.  Cell Rep. 2015 Dec 22;13(11):2425-39. Pubmed


A dynamic protein interaction landscape of the human centrosome-cilium interface
Gupta GD, Coyaud É, Gonçalves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L.  Cell. 2015 Dec 3;163(6):1484-99. Pubmed


High-Resolution CRISPR screens reveal fitness genes and genotype-specific cancer liabilities
Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J.  Cell. 2015 Dec 3;163(6):1515-26. Pubmed |Dataset

*comment in Science


Dependence of human colorectal cells lacking the FBW7 tumor suppressor on the spindle assembly checkpoint
Bailey ML, Singh T, Mero P, Moffat J, Hieter P.  Genetics. 2015 Nov;201(3):885-95. doi: 10.1534/genetics.115.180653. Pubmed


Genome-wide RNAi analysis reveals that simultaneous inhibition of specific mevalonate pathway genes potentiates tumor cell death
Pandyra AA, Mullen PJ, Goard CA, Ericson E, Sharma P, Kalkat M, Yu R, Pong JT, Brown KR, Hart T, Gebbia M, Lang KS, Giaever G, Nislow C, Moffat J, Penn LZ.  Oncotarget. 2015 Sep 29;6(29):26909-21. doi: 10.18632/oncotarget.4817 Pubmed


Selection of recombinant anti-SH3 domain antibodies by high-throughput phage display
Huang H, Economopoulos NO, Liu BA, Uetrecht A, Gu J, Jarvik N, Nadeem V, Pawson T, Moffat J, Miersch S, Sidhu SS.  Protein Sci. 2015 Sep 1. doi: 10.1002/pro.2799. [Epub ahead of print] Pubmed


Identification of P450 Oxidoreductase as a Major Determinant of Sensitivity to Hypoxia-Activated Prodrugs
Hunter FW, Young RJ, Shalev Z, Vellanki RN, Wang J, Gu Y, Joshi N, Sreebhavan S, Weinreb I, Goldstein DP, Moffat J, Ketela T, Brown KR, Koritzinsky M, Solomon B, Rischin D, Wilson WR, Wouters BG.  Cancer Res. 2015 Aug 21. pii: canres.1107.2015. Pubmed


A High Through-put Platform for Recombinant Antibodies to Folded Proteins
Hornsby M, Paduch M, Miersch S, Sääf A, Matsuguchi T, Lee B, Wypisniak K, Doak A, King D, Usatyuk S, Perry K, Lu V, Thomas W, Luke J, Goodman J, Hoey RJ, Lai D, Griffin C, Li Z, Vizeacoumar FJ, Dong D, Campbell E, Anderson S, Zhong N, Gräslund S, Koide S, Moffat J, Sidhu S, Kossiakoff A, Wells J.  Mol Cell Proteomics. 2015 Aug 19. pii: mcp.O115.052209. Pubmed


Genome-wide lentiviral shRNA screen identifies serine/arginine-rich splicing factor 2 as a determinant of oncolytic virus activity in breast cancer cells
Workenhe ST, Ketela T, Moffat J, Cuddington BP, Mossman KL.  Oncogene. 2015 Aug 10. doi: 10.1038/onc.2015.303. Pubmed


Extensive mapping of an innate immune network with CRISPR
Aregger M, Hart T, Moffat J.  Mol Syst Biol. 2015 Jul 24;11(7):821. doi: 10.15252/msb.20156373. Pubmed


Natural Variation in Gene Expression Modulates the Severity of Mutant Phenotypes
Vu V, Verster AJ, Schertzberg M, Chuluunbaatar T, Spensley M, Pajkic D, Hart GT, Moffat J, Fraser AG.  Cell. 2015 Jul 16;162(2):391-402. doi: 10.1016/j.cell.2015.06.037. Pubmed


INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity
Gaudet RG, Sintsova A, Buckwalter CM, Leung N, Cochrane A, Li J, Cox AD, Moffat J, Gray-Owen SD.  Science. 2015 Jun 12;348(6240):1251-5. doi: 10.1126/science.aaa4921. Pubmed


Inhibition of the Mitochondrial Protease ClpP as a Therapeutic Strategy for Human Acute Myeloid Leukemia
Cole A, Wang Z, Coyaud E, Voisin V, Gronda M, Jitkova Y, Mattson R, Hurren R, Babovic S, Maclean N, Restall I, Wang X, Jeyaraju DV, Sukhai MA, Prabha S, Bashir S, Ramakrishnan A, Leung E, Qia YH, Zhang N, Combes KR, Ketela T, Lin F, Houry WA, Aman A, Al-Awar R, Zheng W, Wienholds E, Xu CJ, Dick J, Wang JC, Moffat J, Minden MD, Eaves CJ, Bader GD, Hao Z, Kornblau SM, Raught B, Schimmer AD.  Cancer Cell. 2015 Jun 8;27(6):864-76. doi: 10.1016/j.ccell.2015.05.004. Pubmed


CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae
Koh JL, Chong YT, Friesen H, Moses A, Boone C, Andrews BJ, Moffat J.  G3 (Bethesda). 2015 Apr 15;5(6):1223-32. doi: 10.1534/g3.115.017830. Pubmed


Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis
Chong YT, Koh JL, Friesen H, Duffy K, Cox MJ, Moses A, Moffat J, Boone C, Andrews BJ.  Cell. 2015 Jun 4;161(6):1413-24. doi: 10.1016/j.cell.2015.04.051. Pubmed


RNA Interference Screen to Identify Kinases That Suppress Rescue of ΔF508-CFTR
Trzcińska-Daneluti AM, Chen A, Nguyen L, Murchie R, Jiang C, Moffat J, Pelletier L, Rotin D.  Mol Cell Proteomics. 2015 Jun;14(6):1569-83. doi: 10.1074/mcp.M114.046375. Pubmed


Coiled-coil domain containing 68 (CCDC68) demonstrates a tumor-suppressive role in pancreatic ductal adenocarcinoma
Radulovich N, Leung L, Ibrahimov E, Navab R, Sakashita S, Zhu CQ, Kaufman E, Lockwood WW, Thu KL, Fedyshyn Y, Moffat J, Lam WL, Tsao MS.  Oncogene. 2015 Aug 6;34(32):4238-47. doi: 10.1038/onc.2014.357. Pubmed


Differential regulation of FGFR3 by PTPN1 and PTPN2
St-Germain JR, Taylor P, Zhang W, Li Z, Ketela T, Moffat J, Neel BG, Trudel S, Moran MF.  Proteomics.2015 Jan;15(2-3):419-33. doi: 10.1002/pmic.201400259. Pubmed


2014

A systematic approach to identify novel cancer drug targets using machine learning, inhibitor design and high-throughput screening
Jeon J, Nim S, Teyra J, Datti A, Wrana JL, Sidhu SS, Moffat J, Kim PM.  Genome Med. 2014 Jul 30;6(7):57. doi: 10.1186/s13073-014-0057-7. eCollection 2014. Pubmed


Measuring error rates in genomic perturbation screens: gold standards for human functional genomics
Hart T, Brown KR, Sircoulomb F, Rottapel R, Moffat J.  Mol Syst Biol. 2014 Jul 1;10(7):733. doi: 10.15252/msb.20145216. Pubmed

*comment in MSB


A genome scale overexpression screen to reveal drug activity in human cells
Arnoldo A, Kittanakom S, Heisler LE, Mak AB, Shukalyuk AI, Torti D, Moffat J, Giaever G, Nislow C.  Genome Med. 2014 Apr 29;6(4):32. doi: 10.1186/gm549. eCollection 2014.  Pubmed


The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells
Petschnigg J, Groisman B, Kotlyar M, Taipale M, Zheng Y, Kurat CF, Sayad A, Sierra JR, Mattiazzi Usaj M, Snider J, Nachman A, Krykbaeva I, Tsao MS, Moffat J, Pawson T, Lindquist S, Jurisica I, Stagljar I.  Nat Methods. 2014 May;11(5):585-92. doi: 10.1038/nmeth.2895. Epub 2014 Mar 23. Pubmed


CD133-Targeted Niche-Dependent Therapy in Cancer: A Multipronged Approach
Mak AB, Schnegg C, Lai CY, Ghosh S, Yang MH, Moffat J, Hsu MY.  Am J Pathol. 2014 Feb 28. pii: S0002-9440(14)00078-9. doi: 10.1016/j.ajpath.2014.01.008. [Epub ahead of print]. Pubmed


Post-Translational Regulation of CD133 by ATase1/ATase2-Mediated Lysine Acetylation
Mak AB, Pehar M, Nixon AM, Williams RA, Uetrecht AC, Puglielli L, Moffat J.  J Mol Biol. 2014 Feb 17. pii: S0022-2836(14)00079-5. doi: 10.1016/j.jmb.2014.02.012. [Epub ahead of print]. Pubmed


Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes
Ivarsson Y, Arnold R, McLaughlin M, Nim S, Joshi R, Ray D, Liu B, Teyra J, Pawson T, Moffat J, Li SS, Sidhu SS, Kim PM.  Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2542-7. doi: 10.1073/pnas.1312296111. Epub 2014 Feb 3. Pubmed


The RhoGEF GEF-H1 Is Required for Oncogenic RAS Signaling via KSR-1
Cullis J, Meiri D, Sandi MJ, Radulovich N, Kent OA, Medrano M, Mokady D, Normand J, Larose J, Marcotte R, Marshall CB, Ikura M, Ketela T, Moffat J, Neel BG, Gingras AC, Tsao MS, Rottapel R.  Cancer Cell. 2014 Feb 10;25(2):181-95. doi: 10.1016/j.ccr.2014.01.025.  Pubmed


shRNA kinome screen identifies TBK1 as a therapeutic target for HER2+ breast cancer
Deng T, Liu JC, Chung PE, Uehling D, Aman A, Joseph B, Ketela T, Jiang Z, Schachter NF, Rottapel R, Egan SE, Al-Awar R, Moffat J, Zacksenhaus E.  Cancer Res. 2014 Jan 31. [Epub ahead of print]  Pubmed


2013

A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities
Vizeacoumar FJ, Arnold R, Vizeacoumar FS, Chandrashekhar M, Buzina A, Young JT, Kwan JH, Sayad A, Mero P, Lawo S, Tanaka H, Brown KR, Baryshnikova A, Mak AB, Fedyshyn Y, Wang Y, Brito GC, Kasimer D, Makhnevych T, Ketela T, Datti A, Babu M, Emili A, Pelletier L, Wrana J, Wainberg Z, Kim PM, Rottapel R, O'Brien CA, Andrews B, Boone C, Moffat J.  Mol Syst Biol. 2013 Oct 8;9:696. doi: 10.1038/msb.2013.54.  Pubmed | Supplemental Information | Datasets


Targeting HER2+ breast cancer cells: Lysosomal accumulation of anti-HER2 antibodies is influenced by antibody binding site and conjugation to polymeric nanoparticles
Owen SC, Patel N, Logie J, Pan G, Persson H, Moffat J, Sidhu SS, Shoichet MS.  J Control Release. 2013 Jul 20. doi:pii: S0168-3659(13)00405-7. 10.1016/j.jconrel.2013.07.011. [Epub ahead of print]  Pubmed


Integrated Genomic, Transcriptomic, and RNA-Interference Analysis of Genes in Somatic Copy Number Gains in Pancreatic Ductal Adenocarcinoma
Samuel N, Sayad A, Wilson G, Lemire M, Brown KR, Muthuswamy L, Hudson TJ, Moffat J.  Pancreas. 2013 Aug;42(6):1016-26. doi: 10.1097/MPA.0b013e318287d043.  Pubmed


Miniature shRNA Screens to Characterize Antiproliferative Drugs
Kittanakom S, Arnoldo A, Brown KR, Wallace I, Kunavisarut T, Torti D, Heisler LE, Surendra A, Moffat J, Giaever G, Nislow C.  G3 (Bethesda). 2013 Jun 24. doi:pii: g3.113.006437v1. 10.1534/g3.113.006437. [Epub ahead of print]  Pubmed


RNA interference screens to uncover membrane protein biology
Mak AB, Moffat J.  Brief Funct Genomics. 2013 Sep;12(5):422-9. doi: 10.1093/bfgp/elt022. Epub 2013 Jun 22.  Pubmed


MBNL proteins repress ES-cell-specific alternative splicing and reprogramming
Han H, Irimia M, Ross PJ, Sung HK, Alipanahi B, David L, Golipour A, Gabut M, Michael IP, Nachman EN, Wang E, Trcka D, Thompson T, O'Hanlon D, Slobodeniuc V, Barbosa-Morais NL, Burge CB, Moffat J, Frey BJ, Nagy A, Ellis J, Wrana JL, Blencowe BJ.  Nature. 2013 Jun 5. doi: 10.1038/nature12270. [Epub ahead of print]  Pubmed


A Phosphoproteomics Approach to Identify Candidate Kinase Inhibitor Pathway Targets in Lymphoma-Like Primary Cell Lines
Vojvodic M, Hansford LM, Morozova O, Blakely KM, Taylor P, Fathers KE, Moffat J, Marra M, Smith KM, Moran MF, Kaplan DR.  Curr Drug Discov Technol. 2013 May 20. [Epub ahead of print]  Pubmed


Resveratrol has anti-leukemic activity associated with decreased O-Glcnacylated proteins
Tomic J, McCaw L, Li Y, Hough MR, Ben-David Y, Moffat J, Spaner DE.  Exp Hematol. 2013 Apr 15. doi:pii: S0301-472X(13)00157-4. 10.1016/j.exphem.2013.04.004. [Epub ahead of print]  Pubmed


Scaling up the systematic hunt for mammalian genetic interactions
Hart T, Moffat J  Nat Meth. May;10(5):397-9. doi: 10.1038/nmeth.2449.  Pubmed


Interaction domains of sos1/grb2 are finely tuned for cooperative control of embryonic stem cell fate
Findlay GM, Smith MJ, Lanner F, Hsiung MS, Gish GD, Petsalaki E, Cockburn K, Kaneko T, Huang H, Bagshaw RD, Ketela T, Tucholska M, Taylor L, Bowtell DD, Moffat J, Ikura M, Li SS, Sidhu SS, Rossant J, Pawson T.  Cell. 2013 Feb 28;152(5):1008-1020.   Pubmed


A strategy for modulation of enzymes in the ubiquitin system
Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai AM, Neculai D, Vorobyov A, Garg P, Beatty L, Chan PK, Juang YC, Landry MC, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedadi M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, Rotin D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS.  Science. 2013 Feb 1;339(6119):590-595. Epub 2013 Jan 3.  Pubmed


2012

Regulation of CD133 by HDAC6 Promotes β-Catenin Signaling to Suppress Cancer Cell Differentiation
Mak AB, Nixon AM, Kittanakom S, Stewart JM, Chen GI, Curak J, Gingras AC, Mazitschek R, Neel BG, Stagljar I, Moffat J.   Cell Rep. 2012 Oct 25;2(4):951-63. doi: 10.1016/j.celrep.2012.09.016. Epub 2012 Oct 19.   Pubmed


Suppression of cancer progression by MGAT1 shRNA knockdown
Beheshti Zavareh R, Sukhai MA, Hurren R, Gronda M, Wang X, Simpson CD, Maclean N, Zih F, Ketela T, Swallow CJ, Moffat J, Rose DR, Schachter H, Schimmer AD, Dennis JW.   PLoS One. 2012;7(9):e43721. doi: 10.1371/journal.pone.0043721. Epub 2012 Sep 5.   Pubmed


Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress
Tkach JM, Yimit A, Lee AY, Riffle M, Costanzo M, Jaschob D, Hendry JA, Ou J, Moffat J, Boone C, Davis TN, Nislow C, Brown GW.   Nat Cell Biol. 2012 Jul 29. doi: 10.1038/ncb2549. [Epub ahead of print]   Pubmed


A versatile lentiviral expression system to identify mammalian protein-protein interactions
Mak AB, Moffat J.   Methods. 2012 Jun 17. [Epub ahead of print] Pubmed


Developmentally Programmed Nuclear Destruction during Yeast Gametogenesis.
Eastwood MD, Cheung SW, Lee KY, Moffat J, Meneghini MD.   Dev Cell. 2012 Jul 17;23(1):35-44. Epub 2012 Jun 21. Pubmed


A genome wide shRNA screen identifies α/β hydrolase domain containing 4 (ABHD4) as a novel regulator of anoikis resistance
Simpson CD, Hurren R, Kasimer D, MacLean N, Eberhard Y, Ketela T, Moffat J, Schimmer AD.   Apoptosis. 2012 Jul;17(7):666-78. Pubmed


The mixed lineage leukemia (MLL) fusion-associated gene AF4 promotes CD133 transcription
Mak AB, Nixon AM, Moffat J.   Cancer Res. 2012 Apr 15;72(8):1929-34. Epub 2012 Feb 14.   Pubmed


COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines
Koh JL, Brown KR, Sayad A, Kasimer D, Ketela T, Moffat J.   Nucleic Acids Res. 2012 Jan;40(Database issue):D957-63. Epub 2011 Nov 18.   Pubmed


Analysis of early C2C12 myogenesis identifies stably and differentially expressed transcriptional regulators whose knock-down inhibits myoblast differentiation
Rajan S, Chu Pham Dang H, Djambazian H, Zuzan H, Fedyshyn Y, Ketela T, Moffat J, Hudson TJ, Sladek R.   Physiol Genomics. 2012 Feb 1;44(2):183-97. Epub 2011 Dec 6.  Pubmed


2011

Essential gene profiles in breast, pancreatic, and ovarian cancer cells
Marcotte R, Brown KR, Suarez F, Sayad A, Karamboulas K, Krzyzanowski PM, Sircoulomb F, Medrano M, Fedyshyn Y, Koh JL, van Dyk D, Fedyshyn B, Luhova M, Brito GC, Vizeacoumar FJ, Vizeacoumar FS, Datti A, Kasimer D, Buzina A, Mero P, Misquitta C, Normand J, Haider M, Ketela T, Wrana JL, Rottapel R, Neel BG, Moffat J.   Cancer Discov. 2012 Feb;2(2):172-89. Epub 2011 Dec 29.
Pubmed  | Datasets


CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope
Mak AB, Blakely KM, Williams RA, Penttilä PA, Shukalyuk AI, Osman KT, Kasimer D, Ketela T, Moffat J.   J Biol Chem. 2011 Nov 25;286(47):41046-56. Epub 2011 Sep 21.   Pubmed


Pooled lentiviral shRNA screening for functional genomics in mammalian cells
Blakely K, Ketela T, Moffat J.   Methods Mol Biol. 2011;781:161-82.   Pubmed


A comprehensive platform for highly multiplexed mammalian functional genetic screens
Ketela T, Heisler LE, Brown KR, Ammar R, Kasimer D, Surendra A, Ericson E, Blakely K, Karamboulas D, Smith AM, Durbic T, Arnoldo A, Cheung-Ong K, Koh JL, Gopal S, Cowley GS, Yang X, Grenier JK, Giaever G, Root DE, Moffat J, Nislow C.   BMC Genomics. 2011 May 6;12:213.   Pubmed


Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B
Ni Z, Olsen JB, Guo X, Zhong G, Ruan ED, Marcon E, Young P, Guo H, Li J, Moffat J, Emili A, Greenblatt JF.   Transcription. 2011 Sep-Oct;2(5):237-42.  Pubmed


Inhibition of mitochondrial translation as a therapeutic strategy for human acute myeloid leukemia
Skrtić M, Sriskanthadevan S, Jhas B, Gebbia M, Wang X, Wang Z, Hurren R, Jitkova Y, Gronda M, Maclean N, Lai CK, Eberhard Y, Bartoszko J, Spagnuolo P, Rutledge AC, Datti A, Ketela T, Moffat J, Robinson BH, Cameron JH, Wrana J, Eaves CJ, Minden MD, Wang JC, Dick JE, Humphries K, Nislow C, Giaever G, Schimmer AD.   Cancer Cell. 2011 Nov 15;20(5):674-88.  Pubmed


Inhibition of SREBP1 sensitizes cells to death ligands.
Eberhard Y, Gronda M, Hurren R, Datti A, MacLean N, Ketela T, Moffat J, Wrana JL, Schimmer AD.   Oncotarget. 2011 Mar;2(3):186-96.  Pubmed


2010

SATB2 augments ΔNp63α in head and neck squamous cell carcinoma
Chung J, Lau J, Cheng LS, Grant RI, Robinson F, Ketela T, Reis PP, Roche O, Kamel-Reid S, Moffat J, Ohh M, Perez-Ordonez B, Kaplan DR, Irwin MS.   EMBO Rep. 2010 Oct;11(10):777-83. Epub 2010 Sep 10.  Pubmed


Selective targeting of neuroblastoma tumour-initiating cells by compounds identified in stem cell-based small molecule screens
Smith KM, Datti A, Fujitani M, Grinshtein N, Zhang L, Morozova O, Blakely KM, Rotenberg SA, Hansford LM, Miller FD, Yeger H, Irwin MS, Moffat J, Marra MA, Baruchel S, Wrana JL, Kaplan DR.   EMBO Mol Med. 2010 Sep;2(9):371-84.  Pubmed


A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency
Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Wan C, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J.   Mol Cell Proteomics. 2010 May;9(5):811-23. Epub 2010 Mar 19.  Pubmed


The ubiquitin-activating enzyme E1 as a therapeutic target for the treatment of leukemia and multiple myeloma
Xu GW, Ali M, Wood TE, Wong D, Maclean N, Wang X, Gronda M, Skrtic M, Li X, Hurren R, Mao X, Venkatesan M, Beheshti Zavareh R, Ketela T, Reed JC, Rose D, Moffat J, Batey RA, Dhe-Paganon S, Schimmer AD.   Blood. 2010 Mar 18;115(11):2251-9. Epub 2010 Jan 14.  Pubmed


Selective inhibition of histone deacetylases sensitizes malignant cells to death receptor ligands
Wood TE, Dalili S, Simpson CD, Sukhai MA, Hurren R, Anyiwe K, Mao X, Suarez Saiz F, Gronda M, Eberhard Y, MacLean N, Ketela T, Reed JC, Moffat J, Minden MD, Batey RA, Schimmer AD.   Mol Cancer Ther. 2010 Jan;9(1):246-56. Epub 2010 Jan 6.  Pubmed


2009

Dual regulation by pairs of cyclin-dependent protein kinases and histone deacetylases controls G1 transcription in budding yeast
Huang D, Kaluarachchi S, van Dyk D, Friesen H, Sopko R, Ye W, Bastajian N, Moffat J, Sassi H, Costanzo M, Andrews BJ.   PLoS Biol. 2009 Sep;7(9):e1000188. Epub 2009 Sep 8.  Pubmed


Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning
Jones TR, Carpenter AE, Lamprecht MR, Moffat J, Silver SJ, Grenier JK, Castoreno AB, Eggert US, Root DE, Golland P, Sabatini DM.   Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1826-31. Epub 2009 Feb 2.  Pubmed


2007

Off-target effects associated with long dsRNAs in Drosophila RNAi screens
Moffat J, Reiling JH, Sabatini DM.   Trends Pharmacol Sci. 2007 Apr;28(4):149-51. Epub 2007 Mar 9.  Pubmed


2006

Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1
Guertin DA, Stevens DM, Thoreen CC, Burds AA, Kalaany NY, Moffat J, Brown M, Fitzgerald KJ, Sabatini DM.   Dev Cell. 2006 Dec;11(6):859-71.  Pubmed


CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Carpenter AE, Jones TR, Lamprecht MR, Clarke C, Kang IH, Friman O, Guertin DA, Chang JH, Lindquist RA, Moffat J, Golland P, Sabatini DM.   Genome Biol. 2006;7(10):R100. Epub 2006 Oct 31.  Pubmed


A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen
Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini DM, Root DE.   Cell. 2006 Mar 24;124(6):1283-98.  Pubmed


Building mammalian signalling pathways with RNAi screens
Moffat J, Sabatini DM.   Nat Rev Mol Cell Biol. 2006 Mar;7(3):177-87. Review.  Pubmed


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